Gene description for HUWE1
Gene name HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1
Gene symbol HUWE1
Other names/aliases ARF-BP1
HECTH9
HSPC272
Ib772
LASU1
MRXST
MULE
URE-B1
UREB1
Species Homo sapiens
 Experiment description of studies that identified HUWE1 in Pancreatic duct epithelial cells

1
Experiment ID1660
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SamplePancreatic duct epithelial cells
Sample nameHPDE
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
mRNA
Methods used in the studyRT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
EV-TRACK -

2
Experiment ID1661
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SamplePancreatic duct epithelial cells
Sample nameHPNE
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
mRNA
Methods used in the studyRT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
EV-TRACK -

3
Experiment ID2676
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 35656858   
OrganismHomo sapiens
Experiment descriptionA multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells
AuthorsHinzman CP, Singh B, Bansal S, Li Y, Iliuk A, Girgis M, Herremans KM, Trevino JG, Singh VK, Banerjee PP, Cheema AK.
Journal name J Extracell Vesicles
Publication year2022
SamplePancreatic duct epithelial cells
Sample namehTERT-HPNE
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipid
Metabolite
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

4
Experiment ID2677
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 35656858   
OrganismHomo sapiens
Experiment descriptionA multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells
AuthorsHinzman CP, Singh B, Bansal S, Li Y, Iliuk A, Girgis M, Herremans KM, Trevino JG, Singh VK, Banerjee PP, Cheema AK.
Journal name J Extracell Vesicles
Publication year2022
SamplePancreatic duct epithelial cells
Sample nameHPDE-H6c7
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipid
Metabolite
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

5
Experiment ID2684
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 35656858   
OrganismHomo sapiens
Experiment descriptionA multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells
AuthorsHinzman CP, Singh B, Bansal S, Li Y, Iliuk A, Girgis M, Herremans KM, Trevino JG, Singh VK, Banerjee PP, Cheema AK.
Journal name J Extracell Vesicles
Publication year2022
SamplePancreatic duct epithelial cells
Sample namehTERT-HPNE
Isolation/purification methodsEVtrap
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

6
Experiment ID2685
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 35656858   
OrganismHomo sapiens
Experiment descriptionA multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells
AuthorsHinzman CP, Singh B, Bansal S, Li Y, Iliuk A, Girgis M, Herremans KM, Trevino JG, Singh VK, Banerjee PP, Cheema AK.
Journal name J Extracell Vesicles
Publication year2022
SamplePancreatic duct epithelial cells
Sample nameHPDE-H6c7
Isolation/purification methodsEVtrap
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

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